The Biosynthesis of Glycoproteins
نویسندگان
چکیده
It was previously shown that the particulate fraction of liver was the site of incorporation of glucosamine-l-14C into rat plasma glycoproteins (1). In continuing these studies, we wished to determine whether this incorporation was dependent on the synthesis of protein de nova. Since puromycin inhibits protein biosynthesis (2-5), we have studied the effect of this drug on the incorporation of glucosamine into liver and plasma proteins in viva. Puromycin inhibited the incorporation of glucosamine-r4C into liver and plasma proteins, and there was an accumulation of radioactive glucosamine derivatives in the acid-soluble fraction of the liver. The nature of these compounds has been elucidated.
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